Table 2.
The RNase T1 and A sequence map for T. thermophilus HB27 tRNAASN. Position 7 guanosine, bold and underlined, would be unidentified by using just RNase T1.
| tRNA Sequence | Enzyme | % Coverage | Sequence |
|---|---|---|---|
| (1 – 39) | UCCGCGG[s4U]AGCUCAGCA[Gm]GUAGAGCAGCCGG[Cm]UGUU[t6A]AC | ||
| RNase T1 | 92 | UCCGCG [s4U]AGCUCAGCA[Gm]GUAGAGCAGCCG [Cm]UGUU[t6A]AC | |
| 78 | GCGG[s4U]AGC CAGCA[Gm]GUAGAGCAGC GG[Cm] GU [t6A]AC | ||
|
| |||
| (40 – 76) | CGGUAG[m7G]UCGCAGG[m5s2U]UCG[m1A] GUCCUGCCCGCGGAGCCA | ||
| RNase T1 | CG UAG UCGCAG [m5s2U]UCG[m1A]GUCCUGCCCGCG AGCCA | ||
| RNase A | CGGUAG[m7G]U GCAGG[m5s2U]U G[m1A]GU GCGGAGC | ||