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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Allergy Clin Immunol. 2016 Apr 18;138(3):801–811.e9. doi: 10.1016/j.jaci.2016.02.030

Figure 3. Th2 cytokine production by in vitro expanded SI ILC2.

Figure 3

(A) Flow cytometric gating strategy used to identify in vitro-expanded ILC2 from the SI of naïve mice. (B) Flow cytometric analysis of IL-5, IL-4 and IL-13 expression by Lin− CD45+ GATA-3+ ILC2 in vitro-expanded from the SI of naïve WT, Il13−/−, Il4raF709, Il4raF709 Il1rl1−/− and Il4raF709 Il4−/− mice. (C) Frequencies of IL-5+, IL-4+ and IL-13+ Lin− CD45+ GATA-3+ ILC2 isolated from the SI of the mouse groups from (B). Results represent 3 to 5 mice per group derived from 2 independent experiments. *P < .05, **P < .01, ***P < .001 and ****P < 0.0001 by one-way ANOVA with post-test analysis.