Figure 6. Percentages of CD4⁺ CD25⁺ FOXP3⁺ T_regs in Csf2^−/− exhibit modest reductions in frequency relative to the pool of total CD3⁺ T cell.

PBMCs were obtained from 7 Csf2^−/− FIG mice and 10 FIG mice. (A) Shown are the percentages of CD3⁺ T cells among total leukocytes (excluding granulocytes) and the percentage of CD4⁺ T cells among the total CD3⁺ T cell pool. (B) Shown are the percentages of FSC^low CD4⁺ T cells among the total CD4⁺ T cell pool and the percentages of FSC^low CD8⁺ T cells among the total CD8⁺ T cell pool. (C) Shown are the percentages of CD4⁺ CD25⁺ FOXP3⁺ T_regs among the CD3⁺ T cell pool, the CD3⁺ CD4⁺ T cell pool, and the FSC^high CD3⁺ CD4⁺ T cell pool. t Tests revealed significant differences for all 7 pairwise comparisons (A–C; P < 0.001). These data are representative of 3 independent experiments.