Figure 7. MOG-specific sensitization is deficient in Csf2^−/− mice.

Csf2^−/− and C57BL/6 mice were pretreated with 250 µg PC61 or Y13 mAb on d −4 and −2 and were challenged on d 0 with MOG35–55 in CFA. Ptx was given on d 0 and 2. On d 12, lymphoid cells were isolated from draining nodes; labeled with CellTrace Violet; cultured in triplicate, with or without 1 µM MOG35–55 for 4 d; and analyzed for expression of CD45, CD3, CD4, CD19, and MHC class II. B Cells were gated as the CD19⁺, MHC class II⁺ population. CellTrace Violet dye dilution was used as a measure of proliferation. (A–C) PC61-pretreated Csf2^−/− cultures ± 1 µM MOG35–55 (P < 0.001). Y13-pretreated Csf2^−/− cultures ± 1 µM MOG35–55 (not significant). These data are representative of 3 replicate experiments.