Figure 7. cBiMPS-mediated chemotaxis inhibition is not associated with changes in chemokine receptor expression levels.

Naïve, purified CD4⁺ T cells were incubated with cBiMPS, AzA, or the diluent complete RPMI 1640 medium for 16 h and then examined for expression of CCR7 and CXCR4 by real-time PCR analysis of RNA expression (A) or by flow cytometric intracellular (B) or surface (C) CCR7 protein detection. Means ± sd represent 4 separate experiments, each using T cells pooled from ≥2 mice.