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Primer sequences used in this study
| Genes | Primer sequence (5′–3′) | Product length (bp) | Anneal temp (°C) |
|---|---|---|---|
| PPARγ | Forward: TCCAGCATTTCCACTCCACACT | 207 | 58 |
| Reverse: GAATAAGGCGGGGACACAG | |||
| ADRP | Forward: TTTGCCAGGAAGAATGTGC | 255 | 58 |
| Reverse: TGGTAACCCTCGGATGTTG | |||
| ACTB | Forward: TCTGGCACCACACCTTCT | 120 | 58 |
| Reverse: TGATCTGGGTCATCTTCTCAC |
