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. 2016 Sep 8;7:1391. doi: 10.3389/fmicb.2016.01391

FIGURE 3.

FIGURE 3

(A) Host DNA degradation analysis of ZK126 that was labeled with tritiated thymidine during exponential growth and then infected at 48 h in stationary phase with either T4D or T4 am4332 (DNA polymerase-), in parallel with infection of cultures being tracked in terms of phage production and BS. (B) Status of DNA labeled with tritiated thymidine over the course of similar infections of E. coli B with T4D and T4 amN55x5 (dCMP HMase-) in exponential phase, as reported by Kutter and Wiberg (1968). Both T4 am4332 and T4 amN55x5 produce DNA- phenotype phage when grown on a non-amber suppressor strain such as E. coli B or ZK126. (C) Sucrose gradient analysis determining the size of the acid-insoluble fraction of the host DNA at various times after this exponential phase infection of E. coli B by T4D. “T4” and “T7” refer to phage DNAs used as sedimentation markers.