FIGURE 6.

Leisingera sp. JC1 extracts inhibit V. fischeri in 96-well liquid assays. JC1 cultures were grown at large scale and extracts were prepared using either an indigoidine enriched procedure (igi enriched) or a normal extraction method. All extracts were screened at 500 μg/mL against V. fischeri, V. anguillarum, and V. parahaemolyticus. The JC1 normal extract strongly inhibited growth of V. fischeri (PCA 17.9 ± 9.3) with less activity of the JC1 igi enriched extract against V. fischeri (PCA 63.3 ± 6.7). No inhibition of V. anguillarum or V. parahaemolyticus was observed. Data shown are average PCA values from three experiments (PCA, percent control activity, as compared with DMSO negative control).