FIGURE 8.

Leisingera sp. JC1 upregulates indigoidine production in the presence of V. fischeri. JC1 was cultured alone and in the presence of V. fischeri, V. anguillarum, and V. parahaemolyticus (each vibrio was also monocultured). (A) Extracts were prepared in DMSO at 5 mg/mL for LC–MS (from left to right): JC1 monoculture, JC1 + V. fischeri, JC1 + V. anguillarum, JC1 + V. parahaemolyticus, V. fischeri monoculture, V. anguillarum monoculture, and V. parahaemolyticus monoculture. As shown, the highest pigment production was observed when JC1 was co-cultured with V. fischeri. (B) LC–MS UV chromatograms (299 nm) for all extracts. JC1 indigoidine production was upregulated when co-cultured with V. fischeri (red). Down regulation was observed when co-cultured with V. anguillarum or V. parahaemolyticus (green and pink, respectively). (C) Following extraction, indigoidine production was measured using HPLC and quantitated via measurement of the area under the curve for UV absorption at 299 nm. Fold change was calculated as compared with JC1 monoculture.