TABLE 1 .
Kinetic parameters for the ATP hydrolysis of M-nsp13 with partially duplex RNA substrates with loading strands of various lengthsa
| RNA substrate | Vmax (min−1) | Km (ATP) (mM) |
|---|---|---|
| 5′-RNA-0 (0 ss, 22 ds) | 0.37 ± 0.05 | 1.23 ± 0.49 |
| 5′-RNA-2 (2 ss, 22 ds | 0.47 ± 0.04 | 1.14 ± 0.28 |
| 5′-RNA-5 (5 ss, 22 ds) | 0.72 ± 0.03 | 0.62 ± 0.09 |
| 5′-RNA-10 (10 ss, 22 ds) | 0.99 ± 0.02 | 0.41 ± 0.04 |
| 5′-RNA-20 (20 ss, 22 ds) | 0.99 ± 0.02 | 0.26 ± 0.03 |
a
The Vmax and Km values were determined by fitting the data by nonlinear regression to the Michaelis-Menten equation using Prism 7.0 software. Experiments were performed two times.