TABLE 2 .
Kinetic parameters for M-nsp13 rates of unwinding and amplitudes for the different partially duplex RNA substrates with 5′ overhangs of various lengthsa
| RNA substrate | Amplitude | ku (s−1) |
|---|---|---|
| 5′-RNA-0 (0 ss, 22 ds) | 0.27 ± 0.04 | 0.07 ± 0.03 |
| 5′-RNA-2 (2 ss, 22 ds) | 0.40 ± 0.06 | 0.08 ± 0.03 |
| 5′-RNA-5 (5 ss, 22 ds) | 0.63 ± 0.03 | 0.17 ± 0.03 |
| 5′-RNA-10 (10 ss, 22 ds) | 0.90 ± 0.02 | 0.42 ± 0.02 |
| 5′-RNA-20 (20 ss, 22 ds) | 0.92 ± 0.02 | 1.29 ± 0.14 |
a
The rates of RNA unwinding (ku) and amplitudes were determined by fitting the data by means of nonlinear regression to a single-exponent equation (see Results) using Prism 7.0 software. Experiments were performed two times.