FIG 3.

Quality of preexisting F3-specific CD4 T cells impacts the Ad5-F3 boosting effect. C57BL/6 mice were primed as indicated to generate different qualities of F3-specific CD4 T cells and 6 weeks later boosted by injection of Ad5-F3. Sixteen weeks after the boost, spleens were removed and single-cell suspensions prepared. Cells were incubated with the F3 protein and analyzed by flow cytometry for expression of CD154 or the indicated cytokine (A). Data are means and SEM (n = 5 per group). (B) Relative proportions of CD4 T cells expressing combinations of each cytokineCD154. (C) Cells were incubated with F3 MHC-I peptides and analyzed by flow cytometry for expression of CD107a or the indicated cytokine. Data are means and SEM (n = 5 per group) and are representative of results obtained in 2 or 3 independent experiments. * and **, P < 0.05 and P < 0.01, respectively, for the indicated groups. The actual P value is indicated in comparisons where values of <0.1 were assessed.