Table 1.
Experimental design.
| Groups | 1st week | 2nd-3rd week | 3rd weekend | 4th–6th week | 7th week |
|---|---|---|---|---|---|
| Control (n = 8) |
Behavioral tests | SE (n = 8) |
Sucrose preference & open field test | SE (n = 8) |
Behavioral tests |
| Stress (n = 12) |
Behavioral tests | CUMS (n = 12) | Sucrose preference & open field test | CUMS + water (n = 8) | Behavioral tests |
| Stress + Flu (n = 12) | Behavioral tests | CUMS (n = 12) | Sucrose preference & open field test | CUMS + Flu (n = 8) | Behavioral tests |
| Stress + AS (n = 12) | Behavioral tests | CUMS (n = 12) | Sucrose preference & open field test | CUMS + AS (n = 8) | Behavioral tests |
SE: standard environment; CUMS: chronic unpredictable mild stress; behavioral tests: sucrose preference & open field & forced swim tests.
There are 44 rats in the start of experiment: control (n = 8), stress (n = 12), stress + Flu (n = 12), and stress + AS (n = 12).
The animal number of each group is eight in end stage.