Fig. 1. Concentration-dependent effects of NAC and cysteine on [Ca²⁺]_i in human neutrophils.

(A) NAC (100 nM~1 mM) or (C) cysteine (100 nM~1 mM) was treated at 300 s following 5 min pre-read. [Ca²⁺]_i was measured as described in methods and materials. Changes in [Ca²⁺]_i were expressed as the relative fluorescence intensity of Fluo-3 AM over baseline fluorescence intensity (F/F₀). (B)&(D) [Ca²⁺]_i following treatment of NAC and cysteine was shown as area under curve (AUC), which was calculated for 900 s (300~1200 s) and was expressed as percentage control (% control). Data were analyzed by Graphpad Prism 5.0 (Graphpad software) using ANOVA. Bonferroni test was used for post-hoc comparison. An average of four (A, B) and three (C, D) independent experiments is shown. ns, no significant difference, ^**p<0.01, ^***p<0.001.