Fig. 7. Role of p53 signaling in leptin-induced increase in the expression of the genes related with autophagy.

HepG2 cells were transfected with siRNA targeting p53 or scrambled control followed by incubation with leptin (250 ng/ml) for 48 h. Protein expression level of LC3 II (A), Atg5 (B) and Beclin-1 (C) was determined by Western blot analysis as indicated in the materials and methods. Images are the representative of three separate experiments that showed similar results.