Fig. 1. The effect of DAMGO on resting membrane potential (RMP) in SG neuron.

(A) In current clamp condition, the application of DAMGO (1 µM) caused a reversible hyperpolarization of RMP in SGN (−61.4±1.2 mV, control RMP; −67.6±0.8 mV, DAMGO; n=10). In voltage clamp condition at −60 mV, DAMGO induced an outward current in SG neurons with time course similar to the hyperpolarization (control value of 10.1±4.1 pA and DAMGO 37.8±3.2 pA, n=10, respectively). This experiment was conducted in normal ACSF. (B) The inhibitory effect of naloxone (1 µM) on DAMGO-induced outward current. Data are presented as mean±SEM and asterisks (^*)indicated a significant difference at p<0.05 (paired t-test).