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. 2016 Sep 8;6:32747. doi: 10.1038/srep32747

Figure 3. Stability of trimeric TNF.

Figure 3

(A) TNF-488 (DOL 0.6; 3 ng/mL) was incubated at 37 °C and samples were analyzed by HP-SEC. Column was calibrated using a sample of IgG and Fab fragments (resp. 150 and 50 kDa; grey line, detected at 215 nm). Monomeric TNF was only partially recovered. (B) Disappearance of trimeric TNF-488 (3 ng/mL) in time. (C) TNF-488 (3 ng/mL) was incubated alone or in the presence of 27 or 297 ng/mL of unlabeled TNF for 72 hrs and recovery of trimeric TNF-488 was evaluated as in (A). (D) TNF or TNF-488 (3 ng/mL) was incubated at 37 °C for various times and analyzed using an ELISA that only measures trimeric TNF. (A–C) representative of n = 2; D average of triplicate experiment, error bars indicate SEM. F = fluorescence.