Figure 6. Influence of time, temperature and the TNF inhibitor adalimumab on TNF bioactivity.
(A) Bioactivity of a titration of TNF was measured immediately or after three days of incubation at 4 °C or 37 °C using a TNF sensitive WEHI bioassay. (B) Effect of adalimumab Fab’ on TNF activity, determined immediately or after three days of incubation at 37 °C. (C,D) Effect of adalimumab on TNF activity, measured immediately or after three days of incubation at 4 °C (C) or 37 °C (D). All graphs show representative data of two (A–C) or four (D) experiments.