Figure 6. UCHL5 levels were increased in lung tissues from IPF patients and bleomycin-challenged mice.

(A) Histological image of UCHL5 expressed in non-IPF (“Normal”) and IPF lung tissues. (B) C57BL/6 mice were intranasally challenged with bleomycin (0.045 U/mice) for 3 weeks, and then lung tissues were collected. Lysates from lung tissues were analyzed by immunoblotting with Smad2, Smad3, UCHL5, USP14, and β-actin antibodies. (C) Intensities of blots in the (B) were quantified by imageJ software. Western blot images were cropped to improve the conciseness of the data; samples derived from the same experiment and the blots were processed in parallel.