Figure 2. NFIA is expressed in retinal MG and electroporation of Nfia promotes the formation of radial cells and is sufficient to rescue loss of P27^Kip1 expression resulting from Lhx2 loss of function.

(a,b) Immunohistochemical co-labeling of NFIA with LHX2 at P7 and P14, arrows indicate co-labeled cells. (c–f) Electroporation of Lhx2^+/+ and Lhx2^lox/lox retinas with Cre/GFP/Nfia and analyzed by immunohistochemical co-labeling of GFP with the MG markers P27^Kip1 and GLUL. (g,h) Quantification of GFP/P27^Kip1 and GFP/GLUL co-labeled cells in Lhx2^+/+ and Lhx2^lox/lox mice following Cre/GFP or Cre/GFP/Nfia. (i,j) Quantification of radial cells in Lhx2^+/+ or Lhx2^lox/lox mice following Cre/GFP or Cre/GFP/Nfia electroporation. *Indicates significant decrease (P < 0.05, N = 6 for marker counts, N = 12 for radial morphology counts). Scale bars: 50 um (a–c).