Figure 5. Electroporation of Sox2, Sox8, or Sox9 are insufficient to promote MG development and largely fail to rescue the loss of MG resulting from Lhx2 loss of function, instead promoting amacrine cell development.

(a,b) Quantification of GFP/P27^Kip1 and GFP/GLUL co-labeled cells in Lhx2^+/+ and Lhx2^lox/lox mice following Cre/GFP, Cre/GFP/Sox2, Cre/GFP/Sox8, or Cre/GFP/Sox9 electroporation. (c,d) Quantification of radial cells in Lhx2^+/+ or Lhx2^lox/lox mice following Cre/GFP, Cre/GFP/Sox2, Cre/GFP/Sox8, or Cre/GFP/Sox9 electroporation. (e,f) Quantification of amacrine cells by morphology in Lhx2^+/+ or Lhx2^lox/lox mice following Cre/GFP, Cre/GFP/Sox2, Cre/GFP/Sox8, or Cre/GFP/Sox9 electroporation. *Indicates significant decrease while ^ indicates significant increases (P < 0.05, N = 6 for marker counts and amacrine cell counts, N = 12 for radial morphology counts).