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. 2016 Sep 8;6:32950. doi: 10.1038/srep32950

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Copyright © 2016, The Author(s)

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(a) Purified pyoverdine was loaded into the hole in CAS agar and incubated overnight at 28 °C. (b) Inhibitory concentration (IC₅₀) and (c) lethal dose (LC₅₀) of pyoverdine against Xoo were assayed by measuring absorbance at 600 nm and applying CFU, respectively. (d) Different concentrations of iron (150, 300, 600, 1000 μM FeCl₃) were applied to antagonistic agar plates. (e) Cell growth of P. taiwanensis was detected in 1/2 TSB growth medium with 150, 300, 600 or 1000 μM FeCl₃ or without FeCl₃.