Table 2. Univariate and Multivariate results for Overall Survival.

Parameter	Case-control approach				Case-only approach				External dataset (TCGA)
	Univariate analysis		Multivariate analysis		Univariate analysis		Multivariate analysis		Univariate analysis		Multivariate analysis
	HR (95% CI)	p-value	HR (95% CI)	p-value	HR (95% CI)	p-value	HR (95% CI)	p-value	HR (95% CI)	p-value	HR (95% CI)	p-value
Risk score	2.39 (1.07–5.33)	0.03	2.68 (1.19–5.99)	0.02	2.33 (1.29–4.18)	0.01	2.78 (1.53–5.07)	0.001	2.28 (0.92–5.66)	0.08	1.97 (0.79–4.95)	0.15
Tumor stage	0.40 (0.21–0.78)	0.01	0.50 (0.25–1.01)	0.05	0.40 (0.21–0.78)	0.01	0.46 (0.23–0.93)	0.03	0.32 (0.13–0.78)	0.01	0.29 (0.11–0.74)	0.009
Tumor grade	2.01 (1.04–3.89)	0.04			2.01 (1.04–3.89)	0.04	2.49 (1.26–4.93)	0.01	—	—	—	—
Age at diagnosis	1.06 (1.02–1.09)	0.001	1.05 (1.02–1.09)	0.002	1.06 (1.02–1.09)	0.001	1.05 (1.02–1.09)	0.001	1.03 (1.003–1.06)	0.03	1.03 (1.01–1.06)	0.02
TNBC status	0.99 (0.50–1.95)	0.98			0.99 (0.50–1.95)	0.98			0.63 (0.19–2.12)	0.46

HR = Hazard Ratio; CI = Confidence Interval; TNBC = Triple Negative Breast Cancer.

Risk scores were constructed for the discovery cohort from the three and 14 tRNAs (significant for OS) identified from CC (left panel) and CO (middle panel), respectively. tRNAs significant for OS were validated in TCGA/external dataset (right panel). Patients were dichotomized into low and high-risk groups based on ROC estimated cut-off point. Risk score was significant for univariate and multivariate Cox analysis and patients belonging to high-risk group were associated with poorer OS (HR > 1).