Table 2. Phenotypic properties that separate strain Streptomyces NEAE-82 from related Streptomyces species.
| Characteristic | Streptomyces sp. strain NEAE-82 | Streptomyces fradiae |
|---|---|---|
| Aerial mycelium on ISP mdeium 2 | Reddish brown | Red |
| Substrate mycelium on ISP medium 2 | Not-distinctive | Not-distinctive |
| Production of diffusible pigment | No diffusible pigment | No diffusible pigment |
| Spore chain morphology | Retinaculiaperti including open spirals | Retinaculiaperti morphology, including open spirals |
| Spore surface | Smooth | Smooth |
| Spore shape | Oval to oblong | |
| Melanin production on peptone-yeast extract iron agar (ISP medium 6) | − | − |
| Melanin production on tyrosine agar (ISP medium 7) | − | − |
| Max NaCl tolerance (%, w/v) | 6 | |
| Utilization of carbon sources (1%, w/v) | ||
| D(−) Fructose | + | + |
| D(+) Xylose | + | ± |
| D(+) Galactose | + | |
| D(+) Glucose | + | + |
| L-arabinose | + | + |
| Ribose | + | |
| D(+) Mannose | + | |
| Sucrose | + | ± |
| Maltose | + | |
| Rhamnose | + | ± |
| Raffinose | ± | ± |
| Cellulose | + | |
| Trehalose | + | |
Data for reference species were taken from Bergey’s Manual® of Systematic Bacteriology -volume five the actinobacteria (Goodfellow et al.21).
Abbreviations: +, Positive; −, Negative; ±, Doubtful; Blank cells, no data available.
The optimal growth temperature was 30 °C and optimal pH was 8.5. It exhibited no antimicrobial activities against Staphylococcus aureus, Alternaria solani, Bipolaris oryzae, Sacchromyces cerevisiae, Candida albicans, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Rhizoctonia solani, Fusarium oxysporum, Aspergillus niger and Klebsiella pneumoniae. α–amylase (starch hydrolysis), protease (degradation of casein), cellulase (growth on cellulose), chitosanase and L-asparaginase of strain NEAE-82 were produced while uricase, lecithinase were not produced. Coaggulation and peptonization of milk and gelatin liquefaction were positive.