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| Purification step | Total protein content (mg) | L-asparaginase activity |
|||
|---|---|---|---|---|---|
| Total activity (U) | Specific activity (U/mg protein) | Recovery (%) | Purification fold | ||
| Culture filtrate | 3214.705 | 29507.688 | 9.179 | 100 | 1 |
| (NH4)2SO4, post dialysis | 196.6 | 2557.325 | 13.008 | 8.667 | 1.417 |
| Ion exchange on DEAE Sepharose CL-6B | 47.081 | 1442.393 | 30.636 | 4.888 | 3.338 |
