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. 2016 Sep 8;6:33027. doi: 10.1038/srep33027

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Copyright © 2016, The Author(s)

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(A) Representative images of T- (top) and B- (bottom) cell areas in PPs, pLNs and mLNs, showing Cre-mediated GFP expression in relation to blood and lymphatic vessels. BECs are marked as CD31⁺ cells (blue) and LECs as CD31⁺ Lyve-1⁺ cells (purple). (B) Higher magnification image of a blood vessel (CD31⁺) and GFP⁺ surrounding it. (C) Spleen image showing GFP⁺ cells only in the central part of the white pulp, surrounding endothelial cells (CD31⁺ cells in blue) and co-localizing with collagen IV (red). Scale bar, 75 μm. 5 different mice were analyzed.