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. 2016 Sep 8;6:32885. doi: 10.1038/srep32885

Figure 1. Size of ARSB-null mice, and measurements of arylsulfatase B activity, chondroitin 4-sulfate, and sulfotransferase activity.

Figure 1

(A) Images of the ARSB control and null mice show a significant size disparity. (B) At twelve weeks, ARSB-null mice were significantly smaller than control mice (total n = 17). (C) At twelve weeks, the ARSB-null mice weighed significantly less than the control mice. (D) ARSB activity was significantly less in the ARSB-null mouse hepatic tissue than in the age-matched normal C57BL/6J controls. Values were similar for male and female mice. (E) Consistent with the known reduction in ARSB activity in the hepatic tissue of ARSB-null mice, the total sulfated glycosaminoglycan (GAG) content in the hepatic tissue of the ARSB-null mice was significantly greater than in the controls (n = 20). (F) The chondroitin 4-sulfate (C4S) content was also significantly greater in the ARSB-null mice. The increase in total sulfated GAG was largely attributable to the increase in C4S. (G) In primary hepatocytes from the ARSB null and C57BL/6J control mice, the ARSB activity in the hepatocytes from the ARSB-null mice was significantly less than from the controls (pn = 6). (H) Consistent with the decline in ARSB activity, the total sulfated GAG was markedly increased in the primary hepatocytes from the ARSB-null mice, compared to the normal control (n = 6). (I) Similarly, the C4S in the primary hepatocytes from the ARSB-null mice was significantly greater than in the controls. (J) The mitochondria isolated from the hepatic tissue of the control mice had lower ARSB activity than the primary hepatocytes from the control hepatic tissue (27.6 ± 2.8 nmol/mg protein/h vs. 117.8 ± 6.6 nmol/mg protein/h). In the ARSB null mice, the mitochondrial ARSB activity was virtually absent. (K). The chondroitin 4-sulfate level in the hepatic mitochondria was higher in the ARSB-null mice than in the control mice, but was markedly less than in the primary hepatocytes. (L) Sulfotransferase activity was absent in the mitochondrial preparation from both control and ARSB-null mouse hepatic tissue (n = 3). [ARSB = arylsulfatase B (N-acetylgalactosamine 4-sulfatase); C4S = chondroitin 4-sulfate; c = control; F = female; M = male; null = ARSB-null; ST = sulfotransferase; WT = weight]