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. 2016 Sep 7;15(1):457. doi: 10.1186/s12936-016-1510-4

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Evaluation of overlapping peptides as B cell epitopes. a ELISA was performed for the nine overlapping peptides with protein-immunized sera at a dilution of 1:1000. Reactivity of recombinant protein and pre-immune sera was used as positive and negative control, respectively. b Kinetics of the sera to peptides 3, 5, 6, and 7 with respect to recombinant protein. c ELISA of PfAARP antisera pre-incubated with different concentrations (25, 50 and 100 µg/ml) of PfAARP, peptide 1, peptide 3, peptide 5 and peptide 7 to PfAARP at a dilution of 1:1000. Reactivity of pre-immune sera and immune sera without pre-incubation was used as controls. White bar indicates the reactivity of day 45 anti-PfAARP sera with PfAARP