Table 4.
HFM | LFM | LFM+ Tauro cholate | LFM+ Bovine bile salt | LFM+ Taurine | LFM+ Lecithin | LFM+ Suppl mix | HPS | HPS+ Bovine bile salt | HPS+ Suppl mix | pooled SEM | |
---|---|---|---|---|---|---|---|---|---|---|---|
Total CH (mmol/l) | 10.7a | 8.3a | 8.5a | 8.4a | 8.4a | 9.5a | 24.6b | 8.6a | 8.9a | 24.6b | 1.1 |
VLDL-CHa | 0.1 | 0.1 | 0.2 | 0.2 | 0.2 | 0.1 | 0.5 | 0.2 | 0.1 | 0.5 | |
LDL-CHa | 1.6 | 1.3 | 0.9 | 1.0 | 0.9 | 0.9 | 19.9 | 0.9 | 1.0 | 23.5 | |
HDL-CHa | 10.5 | 7.5 | 6.6 | 7.9 | 6.4 | 8.0 | 7.3 | 7.5 | 5.8 | 9.1 | |
Bile salts (μmol/l) | 31abc | 20bc | 23abc | 48ab | 22bc | 15bc | 55a | 6c | 41ab | 23abc | 7 |
Lathosterol (μg/ml) | 6.6b | 3.8cd | 3.2de | 3.5d | 4.7c | 4.6c | 2.4ef | 5.8b | 3.6d | 2.5ef | 0.4 |
C4 (ng/ml) | 13a | 10a | 10a | 10a | 13a | 11a | 200b | 12a | 9a | 180b | 8 |
Sitosterol(μg/ml) | 67ab | 74a | 47cd | 52bcd | 44d | 64ab | 6e | 64ab | 40d | 6e | 4 |
Campesterol(μg/ml) | 229a | 188ab | 151b | 160b | 133b | 220a | 14c | 239a | 162b | 18c | 16 |
Oxysterols (ng/ml) a | |||||||||||
7α-hydroxy-CH | 124 | 130 | 111 | 116 | 172 | 116 | 1800 | 103 | 591 | 2110 | |
7β-hydroxy-CH | 52 | 37 | 40 | 42 | 66 | 29 | 238 | 32 | 404 | 285 | |
7-keto-hydroxy-CH | 130 | 101 | 122 | 125 | 201 | 59 | 730 | 89 | 1915 | 554 | |
24-hydroxy-CH | 3.6 | 2.2 | 1.8 | 2.0 | 3.3 | 3.5 | 7.1 | 3.8 | 5.3 | 11.5 | |
25-hydroxy-CH | 5 | 5 | 7 | 8 | 9 | 7 | 36 | 5 | 15 | 40 | |
27-hydroxy-CH | 34 | 21 | 14 | 17 | 42 | 22 | 64 | 32 | 22 | 92 |
aLipoprotein and oxysterol profiles were measured in a pooled sample of n = ten animals pr diet group. CV for the different assays, i.e., the analytical variance, as estimated by analyzing a control sample over 10 consecutive days: VLDL-CH: 8.1 %, LDL-CH: 3.4 %, HDL-CH: 5.0 %, VLDL-TAG: 13.1 %, LDL-TAG: 10.5 %, HDL-TAG: 9.7 %. CVs for all oxysterol assays are <8 %, except for 25-hydroxy-CH (11 %) (25). Different letters denote diet groups that are significantly different