Table 2.
Sequences of HHV Primers and Probes
| Sequence Name | Sequence | 5′ Modificationa | 3′ Modification |
|---|---|---|---|
| ERV‐3 probe | Aacatgggagaccaatggccatggg | Quasar 705 | BHQ‐2 |
| ERV‐3 forward | Gttgcttcatgttatgtctgtgg | ||
| ERV‐3 reverse | Ggcggttagtgtgaaattatcttg | ||
| HHV‐1 probe | Atgaccatgtcggtgaccttggc | Cy5 | BHQ‐2 |
| HHV‐1 forward | Ggaacacaaggccaagaaga | ||
| HHV‐1 reverse | Gttgggaacttgggtgtagtt | ||
| HHV‐2 probe | Tcccttcgaggaggtgatcgacaa | FAM | BHQ‐1 |
| HHV‐2 forward | Caccgctactcccagtttatg | ||
| HHV‐2 reverse | Cggtggtctccatgttgtt | ||
| HHV‐3 probe | Tgttatatgacgacaccgtctcgcg | HEX | BHQ‐1 |
| HHV‐3 forward | Gattatgcccgcaaacttgtag | ||
| HHV‐3 reverse | Atggcccgtctattccattc | ||
| HHV‐4 probe | Tccgtcatctccgtcatcaccctc | Quasar 670 | BHQ‐2 |
| HHV‐4 forward | Tagatttgcctccctggtttc | ||
| HHV‐4 reverse | Catctccatcacctccttcatc | ||
| HHV‐5 probe | Tacctggagtccttctgcgagga | CAL Fluor Red 610 | BHQ‐2 |
| HHV‐5 forward | Aaccaagatgcaggtgatagg | ||
| HHV‐5 reverse | Agcgtgacgtgcataaaga | ||
| HHV‐6 probe | Ttattccttcgggtgtgccgtctg | CAL Fluor Red 610 | BHQ‐2 |
| HHV‐6 forward | Atcgaaacgcctacacagaat | ||
| HHV‐6 reverse | Ccagagcggcatcgatattta | ||
| HHV‐7 probe | Gagaacatcgctctaactggatca | FAM | BHQ‐1 |
| HHV‐7 forward | Agcggtacctgtaaaatcatcca | ||
| HHV‐7 reverse | Aacagaaacgccacctcgat | ||
| HHV‐8 probe | Ttggtggtatatagatcaagttc | HEX | BHQ‐1 |
| HHV‐8 forward | Gtccagacgatatgtgcgc | ||
| HHV‐8 reverse | Actccaaaatatcggccgg |
BHQ‐1 indicates Black Hole Quencher 1; BHQ‐2, Black Hole Quencher 2; HHV, human herpesvirus.
a
FAM, HEX, Cy5, Quasar 705, Quasar 670, and CAL Fluor Red 610 are fluorescent dyes.