Fig. 6.

Theanine biosynthesis pathway and the related unigenes. a The glutamine, glutamate, alanine, ethylamine, and theanine concentrations were determined at different stages. The double asterisks indicate a significant difference between the YG or W stages and the G stage (P < 0.01; Student’s t-test). b Theanine biosynthesis pathway. The bracketed numbers in red following each gene name indicate the number of corresponding unigenes identified in our database. c All differentially expressed genes involved in theanine biosynthesis were hierarchically clustered and mapped using the fragments per kilobase of exon per million mapped read values. Colors indicate the normalized signal intensity as defined in the bar