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. 2016 Sep 1;14(7):395–406. doi: 10.1089/adt.2016.730

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© Scott J. Warchal et al., 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Fig. 2. — Phenotypic directions in the first two PCs. Scatter plot of the first two PCs of MDA-MB-231 cells treated with a small compound library. Principal component analysis was carried out on 309 median normalized features extracted from cellular images. Barasertib and Cycloheximide compounds are colored by concentration demonstrating opposite phenotypic directions in PC space producing opposite nuclear phenotypes. Images show nuclei imaged with Hoechst, scale bars: 20 μm. PC, principal component.