Fig. 5.

Heat map and hierarchical clustering of cell lines treated with Saracatinib. (A) Heatmap of TCCS values calculated between all pairs of cell lines treated with Saracatinib with hierarchical clustering by complete linkage of the Euclidean distance. (B) Images demonstrating two similar phenotypic responses—KPL4 and SKBR3—and the dissimilar phenotypic response of MDA-MB-231 cell lines to 1 μM Saracatinib treatment. Channels used: Red—MitoTracker DeepRed (mitochondria); Green—Concanavalin A (endoplasmic reticulum); Blue—Hoechst33342 (nuclei). Scale bar: 100 μm. TCCS, Theta Comparative Cell Scoring.