Table 3.
Stains and Concentrations Used in the Modified Cell-Painting Protocol
| Stain | Structure Labeled | Wavelength (ex/em [nm]) | Concentration | Cat. No.; Supplier |
|---|---|---|---|---|
| Hoechst 33342 | Nuclei | 387/447 | 2 μg/mL | #H1399; Mol. Probes |
| SYTO14 | Nucleoli | 531/593 | 3 μM | #S7576; Invitrogen |
| Phalloidin 594 | F-actin | 562/624 | 0.85 U/mL | #A12381; Invitrogen |
| Wheat germ agglutinin 594 | Golgi and plasma membrane | 562/624 | 8 μg/mL | #W11262; Invitrogen |
| Concanavalin A 488 | Endoplasmic reticulum | 462/520 | 11 μg/mL | #C11252; Invitrogen |
| MitoTracker DeepRed | Mitochondria | 628/692 | 600 nM | #M22426; Invitrogen |