Table. Competence for Zika virus (infection, dissemination and transmission rates)a and Zika virus titres in body, legs plus wings and saliva of Culex pipiens and Aedes aegypti colonies fed orallyb,c.
Days post-infection | Cx. pipiens | Ae. aegypti | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Infection rate | Mean viral titred (SD) in body | Dissemination rate | Mean viral titred in legs + wings |
Transmission rate | Mean viral titred in saliva | Infection rate | Mean viral titred (SD) in body | Dissemination rate | Mean viral titred (SD) in legs + wings |
Transmission rate | Mean viral titred (SD) in saliva | |
0e | 10/10 | 4.23 (0.07) | 0/10 | 0 | 0/10 | 0 | 8/8 | 3.73 (0.18) | 0/8 | 0 | 0/8 | 0 |
3 | 1/10 | 0.17 | 0/10 | 0 | 0/10 | 0 | ND | ND | ND | ND | ND | ND |
7 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 6/12 | 3.76 (1.25) | 6/12 | 2.57 (0.32) | 2/12 | 1.80 (0.14) |
10 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | ND | ND | ND | ND | ND | ND |
14 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 4/8 | 5.12 (0.06) | 4/8 | 3.11 (0.36) | 3/8 | 2.05 (0.97) |
20 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 4/10 | 4.60 (0.21) | 3/10 | 3.08 (0.28) | 3/10 | 2.10 (0.39) |
24 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | ND | ND | ND | ND | ND | ND |
ND: not detected; SD: standard deviation.
a Infection rate: number of virus-positive bodies/number of tested females; dissemination rate: number of virus-positive legs plus wings/number of tested females; transmission rate: number of virus-positive saliva samples/number of tested females.
b The mosquitoes were kept at 26 °C and collected at various days post-infection.
c The viral titre was evaluated by quantitative reverse transcription PCR (qRT-PCR). Crossing point values were compared with a standard curve obtained from 10-fold serial dilutions of virus stock of known concentration [7].
d Expressed as log10 plaque-forming units/mL.
e Immediately after the infectious blood meal.