Table. Competence for Zika virus (infection, dissemination and transmission rates)a and Zika virus titres in body, legs plus wings and saliva of Culex pipiens and Aedes aegypti colonies fed orallyb,c.
| Days post-infection | Cx. pipiens | Ae. aegypti | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Infection rate | Mean viral titred (SD) in body | Dissemination rate | Mean viral titred in legs + wings |
Transmission rate | Mean viral titred in saliva | Infection rate | Mean viral titred (SD) in body | Dissemination rate | Mean viral titred (SD) in legs + wings |
Transmission rate | Mean viral titred (SD) in saliva | |
| 0e | 10/10 | 4.23 (0.07) | 0/10 | 0 | 0/10 | 0 | 8/8 | 3.73 (0.18) | 0/8 | 0 | 0/8 | 0 |
| 3 | 1/10 | 0.17 | 0/10 | 0 | 0/10 | 0 | ND | ND | ND | ND | ND | ND |
| 7 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 6/12 | 3.76 (1.25) | 6/12 | 2.57 (0.32) | 2/12 | 1.80 (0.14) |
| 10 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | ND | ND | ND | ND | ND | ND |
| 14 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 4/8 | 5.12 (0.06) | 4/8 | 3.11 (0.36) | 3/8 | 2.05 (0.97) |
| 20 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | 4/10 | 4.60 (0.21) | 3/10 | 3.08 (0.28) | 3/10 | 2.10 (0.39) |
| 24 | 0/10 | 0 | 0/10 | 0 | 0/10 | 0 | ND | ND | ND | ND | ND | ND |
ND: not detected; SD: standard deviation.
a Infection rate: number of virus-positive bodies/number of tested females; dissemination rate: number of virus-positive legs plus wings/number of tested females; transmission rate: number of virus-positive saliva samples/number of tested females.
b The mosquitoes were kept at 26 °C and collected at various days post-infection.
c The viral titre was evaluated by quantitative reverse transcription PCR (qRT-PCR). Crossing point values were compared with a standard curve obtained from 10-fold serial dilutions of virus stock of known concentration [7].
d Expressed as log10 plaque-forming units/mL.
e Immediately after the infectious blood meal.