Table 1.

List of divergent mutations between primary and metastatic tumors indicating possible clonal evolution. Novel mutations between tumor pairs are highlighted in bold.

Site of novel mutations	Primary site	Mutations in primary	Mutations in metastasis
Metastasis	Breast	PIK3CA:c.1633G>A p.E545K	PIK3CA:c.1633G>A p.E545K TP53:c.853G>A p.E285K
	Breast		PTEN:c.733C>T p.Q245*
	Breast	HRAS:c.34G>A p.G12S	HRAS:c.34G>Ap.G12S SMAD4:c.1553T>C p.I518T
	Breast	PIK3CA:c.1633G>A p.E545K	PIK3CA:c.1633G>A p.E545K PTPN11:c.173A>G p.N58S
	Breast		TP53:c.839G>T p.R280I
	Breast		KRAS:c.34G>C p.G12R
	Breast		KRAS:c.35G>T p.G12V
	Breast		PIK3CA:c.278G>C p.R93P
	Colon	PIK3CA:c.1624G>A p.E542K KRAS:c.35G>T p.G12V	PIK3CA:c.1624G>A p.E542K KRAS:c.35G>T p.G12V SMAD4:c.1081C>A p.R361S
	Colon		PIK3CA:c.1624G>A p.E542K
	Colon	APC:c.3340C>T p.R1114* TP53:c.742C>T p.R248W PIK3CA:c.3140A>Gp.H1047R KRAS:c.436G>A p.A146T	APC:c.3340C>T p.R1114* TP53:c.742C>T p.R248W PIK3CA:c.3140A>G p.H1047R KRAS:c.436G>A p.A146T PIK3CA:c.1030G>A p.V344M APC:c.4348C>T p.R1450*
	Colon	TP53:c.818G>A p.R273H KRAS:c.38G>A p.G13D	TP53:c.818G>A p.R273H KRAS:c.38G>A p.G13D PIK3CA:c.1638G>T p.Q546H
	Colon	KRAS:c.35G>A p.G12D	KRAS:c.35G>A p.G12D TP53:c.844C>T p.R282W PIK3CA:c.1633G>A p.E545K
	Colon	TP53:c.809T>C p.F270S	TP53:c.809T>C p.F270S NRAS:c.183A>C p.Q61H
	Gastric		APC:c.4396G>T p.G1466*
	Lung		FBXW7:c.1436G>A p.R479Q
	Melanoma	CDKN2A:c.158_159delp.M53fs NRAS:c.181C>A p.Q61K	CDKN2A:c.158_159del p.M53fs NRAS:c.181C>A p.Q61K CTNNB1:c.133T>C p.S45P
	Renal cell		TP53:c.742C>T p.R248W
	Renal cell		TP53:c.581T>G p.L194R
	Sarcoma		FBXW7:c.1698G>A p.W566*
Primary	Breast	TP53:c.602T>A p.L201*
	Breast	TP53:c.526T>Ap.C176S TP53:c.581T>G p.L194R	TP53:c.526T>A p.C176S
	Breast	PIK3CA:c.1624G>A p.E542K
	Breast	TP53:c.722C>A p.S241Y ABL1:c.844G>C p.E282Q PIK3CA:c.1633G>A p.E545K PTEN:c.511C>T p.Q171*	TP53:c.722C>A p.S241Y ABL1:c.844G>C p.E282Q PIK3CA:c.1633G>A p.E545K
	Colon	KRAS:c.35G>A p.G12D FBXW7:c.1375G>A p.G459R APC:c.2626C>T p.R876*	KRAS:c.35G>A p.G12D FBXW7:c.1375G>A p.G459R
	Colon	APC:c.4495G>T p.G1499* NRAS:c.35G>T p.G12V PIK3CA:c.1637A>G p.Q546R TP53:c.581T>A p.L194H	APC:c.4495G>T p.G1499* NRAS:c.35G>T p.G12V PIK3CA:c.1637A>G p.Q546R
	Colon	APC:c.3883G>T p.E1295* TP53:c.817C>T p.R273C SMAD4:c.1082G>A p.R361H	APC:c.3883G>T p.E1295* TP53:c.817C>T p.R273C
	Colon	TP53:c.527G>A p.C176Y KRAS:c.38G>A p.G13D SMAD4:c.1529G>T p.G510V	TP53:c.527G>A p.C176Y KRAS:c.38G>A p.G13D
	Melanoma	MLH1:c.1151T>A p.V384D NRAS:c.182A>G p.Q61R KIT:c.1621A>C p.M541L	MLH1:c.1151T>A p.V384D NRAS:c.182A>G p.Q61R
	Melanoma	BRAF:c.1406G>A p.G469E KRAS:c.40G>A p.V14I	BRAF:c.1406G>A p.G469E
	Sarcoma	TP53:c.535C>A p.H179N
	Thyroid	BRAF:c.1799T>A p.V600E AKT1:c.49G>A p.E17K	BRAF:c.1799T>A p.V600E
Both	Small intestine	TP53:c.856G>A p.E286K ERBB2:c.2524G>A p.V842I	TP53:c.856G>A p.E286K ERBB4:c.2817G>T p.L939F SMAD4:c.380G>T p.C127F KRAS:c.35G>A p.G12D
	Breast	TP53:c.626_627del p.R209fs	TP53:c.524G>A p.R175H
	Colon	KRAS:c.35G>T p.G12V TP53:c.273G>A p.W91*	KRAS:c.35G>T p.G12V TP53:c.725G>A p.C242Y PIK3CA:c.3140A>G p.H1047R
	Colon	PIK3CA:c.263G>A p.R88Q KRAS:c.38G>A p.G13D	PIK3CA:c.263G>A p.R88Q GNAS:c.602G>A p.R201H
	Colon	APC:c.3927_3931delp.E1309fs KRAS:c.35G>T p.G12V	APC:c.3927_3931delp.E1309fs PIK3CA:c.263G>A p.R88Q
	Sarcoma	TP53:c.818G>A p.R273H CSF1R:c.950A>G p.E317G	TP53:c.844C>T p.R282W TP53:c.871A>G p.K291E CSF1R:c.923A>G p.N308S