Figure 1. PBMCs from adult donors contain more pTFH cells than UCB.
Mononuclear cell fractions were isolated from normal UCB and normal PB samples and stained with CD3, CD4, and CXCR5. pTFH CD4+CXCR5+ populations were gated from the Live CD3+ population in each sample. A) Example gating for quantification of pTFH CD4+CXCR5+ populations in an UCB mononuclear cell fraction (left) and a PBMC (right). B) Percentage of pTFH CD4+CXCR5+ cells found in UCB (n=6) versus PBMC (n=6). **p=0.002, Mann-Whitney t-test.