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. Author manuscript; available in PMC: 2016 Sep 8.
Published in final edited form as: Biol Blood Marrow Transplant. 2016 Jan 13;22(5):825–833. doi: 10.1016/j.bbmt.2016.01.003

Figure 3. MRD and UCB pTFH cells possess a central memory cell phenotype.

Figure 3

(A) Representative gating path and characterization of pTFH cells following MRD HCT at D60. (B-E) At D60 and 1 year post transplant, CD4 T cells from peripheral blood were gated from the Live CD3+ population. Subsequently, CXCR5+ (2B, 2D) and CXCR5− (2C, 2E) populations within the CD4+ subset were analyzed for CD45RO, CD27, and CD57 expression. Populations identified: naïve (CD45RO−CD27+), central memory (CD45RO+CD27+), effector memory (CD45RO+CD27−), effector (CD45RO+CD57+), and terminal effector (CD45RO−CD57+). In both MRD and UCB recipients, the majority of CXCR5+ cells are central memory cells at day 60 (3B) and 1 year (3C). CXCR5− cells were predominantly naïve, central memory and effector memory CD4 T cells at both 60 days (3D) and 1 year (3E).