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. Author manuscript; available in PMC: 2016 Sep 8.
Published in final edited form as: Biol Blood Marrow Transplant. 2016 Jan 13;22(5):825–833. doi: 10.1016/j.bbmt.2016.01.003

Figure 4. pTFH cells from both UCB and MRD recipients produce multiple cytokines in response to T cell receptor stimulation.

Figure 4

Representative gating path and characterization of pTFH cells stimulated with SEB following MRD HCT at 1 year. CD4 T cells from peripheral blood were gated from the live CD3+ population. Subsequently, CXCR5+ and CXCR5− populations within the CD4+ subset were analyzed for expression of CD40L and multiple cytokines including IL-21, IL-2, IL-17, IFNγ, and TNFα. Graphs B-F show compiled data for CXCR5+ cells that coexpress CD40L and B) IL-21, C) IL-2, D) IL-17, E) IFNy, or F) TNFα. Differences between donors (B-F) were non-significant.