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. 2016 Jun 15;36(24):6431–6444. doi: 10.1523/JNEUROSCI.0419-16.2016

Figure 5.

Figure 5.

Expression of ABHD17 selectively depalmitoylates PSD-95 in neurons. Neurons infected with AAV vectors, mock (−), wild-type (WT) ABHD17B, or inactive ABHD17B-D235A mutant (D235A), were processed for the APEGS assay. The obtained PEGylated samples were subjected to WB with indicated antibodies. Note that ABHD17B robustly depalmitoylated PSD-95 in neurons. Closed and open arrowheads indicate the positions of palmitoylated and non-palmitoylated proteins, respectively. Palm*, the number of palmitoylation sites is not determined. We found that mGluR5 was palmitoylated at one specific site (data not shown). GABAARγ2, GABAA receptor γ2.