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. 2016 Jun 15;36(24):6431–6444. doi: 10.1523/JNEUROSCI.0419-16.2016

Figure 7.

Figure 7.

Unique dynamic palmitate turnover on PSD-95. A–C, To monitor the depalmitoylation process of PSD-95, hippocampal neurons were treated with 2-bromopalmitate (2-BP) for the indicated periods and subjected to the APEGS assay, followed by WB with anti-PSD-95 antibody. B, A graph showing that the depalmitoylation kinetics of PSD-95 decelerated as neurons developed. C, The half-life of palmitate on PSD-95 was prolonged as neurons developed from 7 to 21 DIV. *p < 0.05, **p < 0.01 by one-way ANOVA with post hoc Dunnet's test; n = 3 independent cultures. D, Rapid palmitate turnover was observed only in PSD-95, but not in GluA1, GluN2A, mGluR5, Gαq, and HRas in 7 and 21 DIV neurons. Closed and open arrowheads indicate the positions of palmitoylated and non-palmitoylated proteins, respectively. ND, Not determined due to the low expression of GluN2A. n = 2–4 independent cultures.