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. 2016 Jun 15;36(24):6431–6444. doi: 10.1523/JNEUROSCI.0419-16.2016

Figure 8.

Figure 8.

ABHD17 specifically mediates PSD-95 depalmitoylation in neurons. A–D, Triple knockdown of ABHD17 members (miR-ABHD17s) in rat hippocampal neurons significantly delayed PSD-95 depalmitoylation process (A, B, D). A graph showing that ABHD17 knockdown caused the increase in the basal level of palmitoylation stoichiometry of PSD-95 (C; n = 5 independent cultures) and prolonged the half-life (t1/2) of palmitoylated PSD-95 (D; n = 4 independent cultures). E, F, ABHD17 knockdown inhibited 2-BP-induced PSD-95 declustering. Scale bar, 5 μm. n = 3 independent experiments (9 neurons). G, H, ABHD17 knockdown did not affect GluA1 depalmitoylation process (G), and the basal levels of the palmitoylation stoichiometry of GluA1 (mono-palmitoylated; n = 5), Gαq (n = 4), and HRas (n = 3) (H). *p < 0.05, **p < 0.01 by the Student's t test. n.s., Not significant. Closed and open arrowheads indicate the positions of palmitoylated and non-palmitoylated proteins, respectively (A, G).