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. 2016 Sep 2;12:1744806916666841. doi: 10.1177/1744806916666841

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Effect of tumor injection into the V3 division of the TG (tongue) on mechanical allodynia in the V2 division (vibrissal pad). Mice were tested with von Frey filaments applied to the vibrissal pad with detection of escape responses at nine days after tumor injection. Data are plotted as % response to a series of five applications of a given filament. (a). Comparison of mice with an HSC2 tumor in the tongue compared to an untreated control group. (b) Evaluation of the effect of inoculation of the colon cancer cell line HT29 with untreated control mice. (c) Effect of treatment with morphine 3 mg/kg i.p. 30 min prior to testing in HSC2 tumor-or untreated control mice. (e) and (f) Effect of tramadol 20 mg/kg i.p.in HSC2 tumor (Panel e) or untreated control (Panel f) mice. Data are mean ± SEM. n = 8–12 per group.