Fig 3. Heat shock induces some developmentally regulated mRNAs.
The Venn diagrams are constructed such that all areas are proportional to the numbers of genes included. Ratios for salivary gland transcriptomes were calculated using the raw data from [28] and [29]. Categories are: (i) 246 mRNAs that increased in abundance after heat shock (Padj <0.01 and 39/27 >2, pale cyan ellipse); (ii) 1775 mRNAs that are >2x more abundant in salivary gland (SG) trypanosomes than in procyclic form (PC) trypanosomes (SG>2XPC, yellow); (iii) 2509 mRNAs that are >2x more abundant in salivary gland (SG) trypanosomes than in bloodstream form (BS) trypanosomes (SG>2XBS, orange); (iv) 985 mRNAs that are >2x more abundant in bloodstream form (BS) trypanosomes than in procyclic form (PC) trypanosomes [29] (BS>2xPC, pink); (v) mRNAs that decreased in abundance after heat shock (Padj <0.01 and 39/27 <0.5, labelled as 27>39, violet); (vi) 582 mRNAs that are >2x less abundant in salivary gland (SG) trypanosomes than in procyclic form (PC) trypanosomes (PC>2xSG, dark green), and (vii) 872 mRNAs that are >2x less abundant in bloodstream form (BS) trypanosomes than in procyclic form (PC) trypanosomes [29] (PC>2xBS, pale green). (A) The mRNAs that increased in abundance after heat shock (cyan) were compared with mRNAs that are more abundant in salivary gland trypanosomes than in procyclic form trypanosomes (yellow), and mRNAs that are more abundant in salivary gland trypanosomes than in bloodstream form trypanosomes (orange). The overlap between the last two categories indicates the 906 mRNAs that show the highest abundance in salivary gland trypanosomes. Of these, 81 were also increased by heat shock. The probability that the overlaps would arise by chance (Fisher test) are indicated using blue arrows. The probability of the overlap between 39>27 and SG>2xBS was 6 x 10−27; the probability of the overlap between 39>27 and SG>2xBS was 6 x 10−5; and the probability of the overlap between all three was 9 x 10−16. (B) The mRNAs that increased in abundance after heat shock (cyan) were compared with mRNAs that are more abundant in salivary gland trypanosomes than in procyclic form trypanosomes (yellow), and with mRNAs that are abundant in bloodstream form trypanosomes than in procyclic form trypanosomes (pink). Fischer tests are for the overlaps between 39>27 and either BS>2xPC or SG>2XPC. (C) The mRNAs that decreased in abundance after heat shock (violet) were compared with mRNAs that are less abundant in salivary gland trypanosomes than in procyclic form trypanosomes (dark green), and with mRNAs that are less abundant in bloodstream form (BS) trypanosomes than in procyclic form trypanosomes (pale green).