Skip to main content
. 2016 Sep 8;10(9):e0004982. doi: 10.1371/journal.pntd.0004982

Fig 4. Confirmation of results for individual mRNAs.

Fig 4

(A) Extracts from cells grown only at 27°C, or subjected to a 39°C heat shock, were separated on sucrose gradients: the absorbance profiles of two typical gradients are shown. (B) Northern blots of chosen mRNAs (gene IDs on the right) that moved from the non-polysomal to the polysomal fraction after heat shock. A typical rRNA profile is shown as a control. In vitro transcribed human β-globin RNA was added to each fraction before RNA preparation, and is shown as a control of equal RNA isolation efficiency. (C) ZC3H11, PIP39 and RBP6 protein levels before and after chronic mild heat shock (16h at 37°C) analyzed by Western blotting. An unspecific band (*) recognized by the anti-ZC3H11 antibody is shown as loading control.