Table 3. Antibody panels for FACS.
| Antigen | Clone | Isotype | Fluorochrome | Labelling | Source of antibody | |
|---|---|---|---|---|---|---|
| White blood cells | Triple staining | |||||
| CD3 | PPT3 | IgG1 | APC | Secondary1 | Southern Biotech | |
| CD4 | 74-12-4 | IgG2b | FITC | Secondary1 | Southern Biotech | |
| CD8α | 76-2-11 | IgG2a | PE | Secondary1 | Southern Biotech | |
| Single staining | ||||||
| CD172a | 74-22-15 | IgG2b | FITC | Secondary1 | VMRD | |
| CD21 | B6-11C9 | IgG1 | APC | Secondary1 | Southern Biotech | |
| BALF cells | Triple staining | |||||
| CD3 | PPT3 | IgG1 | APC | Secondary1 | Southern Biotech | |
| CD4 | 74-12-4 | IgG2b | FITC | Secondary1 | Southern Biotech | |
| CD8α | 76-2-11 | IgG2a | PE | Secondary1 | Southern Biotech | |
| Double staining | ||||||
| CD172a | 74-22-15 | IgG2b | PE | Secondary1 | VMRD | |
| CD172a | 74-22-15 | IgG1 | PE | Secondary1 | Beckman Coulter | |
| CD 14 | MIL2 | IgG2b | FITC | Primary | BioSource | |
| SLAII-DR | 2E9/13 | IgG2b | FITC | Primary | Serotec | |
| CD163 | 2a10/11 | IgG1 | FITC | Primary | Serotec | |
| TLR4 | IgM | FITC | Secondary1 | Gift by J. Dominguez | ||
| Single staining | ||||||
| Granulocytes | 2B2 | IgG1 | FITC | Serotec |
1: For secondary labelling cells were incubated with isotype specific secondary antibodies (goat anti–mouse, source Southern Biotech)