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. 2016 Sep 8;12(9):e1005877. doi: 10.1371/journal.ppat.1005877

Fig 3. Quantification of the number of neurons expressing the lytic and/or the latent transcriptional program markers.

Fig 3

A) Quantification of neurons positive for viral protein at 40 hrs pi. This experiment included 17LATpVP16R, the genomic rescue of 17LATpVP16, as well as 17LATpICP0 and two mutants made on the LAT null background parent 17AH (17AHLATpLacZ and 17AHLATpICP0). There was no significant difference in the number of neurons positive for viral proteins [11] in whole TG except for the TG infected with 17LATpVP16, which contained significantly more positive neurons (p<0.0004, Fisher’s exact test). B) Quantification of neurons positive for viral protein, LATp activity, or both at 46 hrs pi. Each point represents the number of positive neurons in a TG. C) TG were fixed and processed for the simultaneous detection of b-gal activity (blue neurons:red arrows) and viral protein expression (brown neurons:black arrows). Shown are representative photomicrographs of whole ganglia at the same magnification. The number of neurons in TG expressing either viral proteins (lytic), LacZ from the LAT promoter (“latent”), or both (lytic+“latent”) were enumerated (shown in B).