Figure 4.

AOX partially rescues cleft thorax and developmental lethality of tubGS/RU486. Proportion of adult progeny exhibiting the indicated phenotypes, with hemizygous transgenes as indicated, cultured with (+) or without (–) 10 μM RU486. n ≥ 3 replicate vials for each genotype studied (except UAS-Ndi, n = 2, hence no error bars shown). Transgenic lines containing tub-AOX transgenes (Kemppainen et al. 2014) had either a single hemizygous copy or else five copies (two homozygous, plus hemizygous copy on chromosome 3, combined with tubGS on the same chromosome). * denotes data classes significantly different from the equivalent class for control lacking any transgene additional to tubGS (Student’s t-test with Bonferroni correction, P < 0.01). (B) Proportion of pupae from two different genetic backgrounds (bkd), as shown, eclosing after culture in 10 μM RU486. All pupae carried the tubGS driver and either no other transgene, or either of two different UAS-AOX transgenes, as indicated. # and * denote data classes significantly different from nontransgenic flies in the same genetic background (Student’s t-test, P < 0.05 or 0.01, respectively). (C) Proportion of pupae eclosing as viable or nonviable adults after culture in 10 μM RU486. All pupae carried the tubGS driver and either no other transgene, or else five copies of tub-AOX transgenes (see above). Nonviable adults were those that died on the day of eclosion. * denotes phenotypic classes of transgenic flies significantly different from corresponding class of nontransgenic flies (Student’s t-test, P < 0.01). AOX, alternative oxidase; GFP, green fluorescent protein; tubGS; α-tubulin-GeneSwitch.