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. 2016 Jul 22;6(9):2971–2981. doi: 10.1534/g3.116.033118

Figure 7.

Figure 7

Evidence that the short and long CUT isoforms of EUC1 initiate from the same transcription start sites. (A) Primer extension analysis of the 5′ ends of EUC1 transcripts produced in strains that express the EUC1 SC transcript (rrp6∆, YJ746) or both the EUC1 SC and LC transcripts (paf1∆ rrp6∆, KY2729). The pEUC1∆2 mutant (YJ1130) was used as a negative control as this strain displays severely reduced EUC1 transcription. A DNA sequencing ladder is shown on the left. (B) A schematic diagram of the ECM3 locus with the positions of the upstream EUC1 CUTs and the pEUC1∆1 and pEUC1∆2 mutations indicated. For simplicity, the EUC1 SC and LC isoforms are diagrammed as initiating at a single start site to reflect that the closely positioned start sites detected in (A) do not appear as distinct isoforms by northern blot analysis. CUT, cryptic unstable transcript; LC, long CUT; SC, short CUT; PIC, preinitiation complex.