Table 2. Potential heterogeneous sites identified within peptides of MS detected T. pallidum proteins.
| Amino acid detected at site 'X' in peptide sequence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MALDI TOF/TOF | ESI-LTQ-Orbitrap | |||||||||
| Sample | Sample | |||||||||
| UniProt Accession Number# | TP number | Protein Name | Molecular Weight (kDa) | Identified Peptide Sequence | TPA-A | TPB-B | TPC-C | TPA-A | TPB-B | TPC-C |
| O88098 | TP0082 | Formate hydrogenlyase transcriptional activator (FhlA) | 66 | (R)LYPIXNAR(K) | T | D | ||||
| O83276 | TP0248 | Uncharacterized lipoprotein | 15 | (R)AYELXER(S) | T | H | ||||
| O83690 | TP0692 | Protein RecA | 44 | (K)TLRRXASR(G) | Y | H | T | P | ||
| O83892 | TP0922 | Uncharacterized protein | 33 | (R)ALXGNDPSAAVR(V) | V | A | ||||
Legend:
#: UniProt Proteome ID: UP000014259; Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF/TOF); electrospray ionization LTQ-Orbitrap tandem mass spectrometry (ESI-LTQ-Orbitrap), X = undetermined amino acid site